论文部分内容阅读
目的研究葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的体外细胞毒性。方法采用四甲基偶氮唑蓝(MTT)法检测葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH的细胞毒性;倒置显微镜观察细胞形态;流式细胞仪定量分析细胞周期。结果作用48 h后,葫芦素B以剂量依赖方式抑制SK-N-SH细胞的生长,0.143和0.716μmol.L-1葫芦素B固体脂质纳米粒对SK-N-SH细胞的抑制率分别为(34.9±4.6)%和(53.6±6.3)%,与对应浓度的葫芦B原料组的(13.2±2.3)%和(43.4±5.5)%相比较有显著性差异(P<0.05)。葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的IC50为0.508μmol.L-1,而葫芦素B原料为12.6μmol.L-1。细胞形态观察结果显示,与二甲基亚砜(DMSO)组相比较,葫芦素B原料及葫芦素B固体脂质纳米粒组的细胞密度下降,细胞体积缩小,大多数细胞形状发生改变。流式细胞仪检测结果显示,葫芦素B原料可将SK-N-SH细胞抑制在S期和G2/M期,而葫芦素B固体脂质纳米粒可把SK-N-SH抑制在G2/M期。结论葫芦素B固体脂质纳米粒与葫芦素B原料药既能抑制人神经母细胞瘤SK-N-SH细胞增殖,又能诱导其凋亡,显示出较强的细胞毒性,且前者毒性更强。
Objective To study the in vitro cytotoxicity of cucurbitacin B solid lipid nanoparticles on human neuroblastoma SK-N-SH cells. Methods Cytotoxicity of cucurbitacin B solid lipid nanoparticles to human neuroblastoma SK-N-SH was detected by MTT method. The morphological changes of cells were observed under inverted microscope. The cell viability was analyzed by flow cytometry cycle. Results After 48 h, cucurbitacin B inhibited the growth of SK-N-SH cells in a dose-dependent manner. The inhibitory rates of cucurbitacin B solid-lipid nanoparticles at 0.143 and 0.716 μmol.L-1 on SK-N-SH cells were (34.9 ± 4.6)% and (53.6 ± 6.3)%, respectively, compared with that of (13.2 ± 2.3)% and (43.4 ± 5.5)% of the corresponding concentrations of gourd B (P <0.05). The cucurbitacin B solid lipid nanoparticles had an IC50 of 0.508 μmol.L-1 for human neuroblastoma SK-N-SH cells and 12.6 μmol.L-1 for cucurbitacin B. Cell morphology observation showed that compared with dimethyl sulfoxide (DMSO) group, the cell density of cucurbitacin B group and cucurbitacin B solid lipid nanoparticle group decreased, the cell volume decreased, most of the cell shape changed. Flow cytometry showed that cucurbitacin B inhibited SK-N-SH cells in S phase and G2 / M phase, whereas cucurbitacin B solid lipid nanoparticles inhibited SK-N-SH in G2 / M period. Conclusion Cucurbitacin B solid lipid nanoparticles and cucurbitacin B can not only inhibit the proliferation of human neuroblastoma SK-N-SH cells, but also induce apoptosis of the neuroblastoma cell line SK-N-SH, showing strong cytotoxicity Strong.