论文部分内容阅读
在前期已获得红麻atp9基因编码区的基础上,以红麻细胞质雄性不育系P3A、保持系和F1代为材料,随机选取不同材料的3个atp9独立gDNA克隆子进行测序和序列比对。结果显示:具有不育细胞质的不育系和F1代与具有可育细胞质的保持系在第290位点处存在碱基差异,具有不育细胞质的材料均为碱基T,而具有可育细胞质的材料在相应位点为碱基A;atp9基因第290位点处T/A碱基转换的cSNP位点经已知细胞质的红麻种质资源的进一步检测,同时结合这些资源的田间育性进行分析发现,红麻种质资源UG93-2MS-1、UG93-2MS-2、UG93-2MS-3、UG93-2-22、KN250、KN142、ZB90在第290位点处为碱基T,能扩增出319bp条带,在田间对应的育性除品种UG93-2-22为可育外,其余均为不育或半不育;而福红992在第290位点处为碱基A,未能扩增出319bp条带,在田间对应的育性为可育。因此,位于atp9基因的第290位点处的T/A碱基转换的cSNP位点与红麻不育细胞质相关或连锁,为红麻不育细胞质鉴定提供了新的检测手段。
Based on the coding region of atp9 gene of kenaf in the early stage, three atp9 independent gDNA clones with different materials were randomly selected from the cytoplasm male sterile line P3A, maintainer line and F1 generation of kenaf for sequencing and sequence alignment. The results showed that there was a base difference at the 290th position between male sterile line and sterile F1 line and male sterile line with sterile cytoplasm. The material with sterile cytoplasm was base T, but the cytoplasm with fertile cytoplasm The site of the atp9 gene at position 290 was further detected by the known cytoplasmic kenaf germplasm resources, and at the same time the cSNP loci of the T / A base conversion were combined with the field fertility of these resources The results showed that the kenaf germplasm resources UG93-2MS-1, UG93-2MS-2, UG93-2MS-3, UG93-2-22, KN250, KN142 and ZB90 were T at the 290th position The 319bp band was amplified, and the corresponding fertility in the field was fertile except for the variety UG93-2-22, while the rest were sterile or semi-sterile. The Fuhong 992 was a base A at the 290th position, The 319 bp band was not amplified, and the corresponding fertility in the field was fertile. Therefore, the T / A base-converted cSNP site located at the 290th site of the atp9 gene is related or linked to kenaf infertility cytoplasm, which provides a new detection method for kenaf infertility cytoplasm identification.