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应用显微外科技术剥除人胚肌腱外膜组织后，经过胰蛋白酶及胶原酶分步消化分离出人胚腱细胞，使用含２０％新生小牛血清的Ｆ－１２培养液将其传代培养１５代后全部冻存。该细胞贴壁生长，具有接触抑制的性质。细胞群体倍增时间大致为４天与细胞有丝分裂高峰时间相符。在传代培养过程中探索了体外培养的腱细胞生长的最适宜条件。通过冻存后复苏的细胞继续培养发现，冻存对其生长、形态、遗传学等特征无明显影响。本研究测定了培养细胞分泌羟脯氨酸量，提示培养的人胚腱细胞与体内腱细胞同样具有合成胶原的能力，并且这种能力不为传代所改变。
After removal of the adventitia of human embryo tendon by microsurgical technique, human embryonic tenocytes were digested by trypsin and collagenase and cultured in F-12 medium containing 20% fetal bovine serum All frozen after generation. The cells grow adherently and have contact inhibition properties. Cell population doubling time is roughly 4 days consistent with peak cell mitosis. In the subculturing process to explore the most suitable conditions for the growth of tendon cells cultured in vitro. After cryopreservation and recovery of cells continue to cultivate, cryopreservation of its growth, morphology, genetics and other features no significant effect. The present study measured the amount of hydroxyproline secreted by cultured cells, suggesting that cultured human embryonic tendon cells have the same ability to synthesize collagen as in vivo tendon cells, and that this ability is not altered by passage.