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14只日本大耳白雄兔,7只为假手术组(SOG),7只为输精管结扎组(VG)。16个月后,检测前列腺睾酮受体和血清睾酮的变化。结果是VG和SOG胞液受体的kd值分别为10.21nM和3.42nM,最大结合容量分别为33.68fmol/mgpro.和10.42fmol/mg pro;胞核受体的kd值分别为9.36nM和4.81nM,最大结合容量分别为440.90fmol/mg DNA和203.66fmol/mg DNA。单点分析的结果是VG和SOG胞液受体浓度分别为26.13±5.64和8.40±1.66fmol/mg pro,VG明显高于SOG(P<0.01);胞核受体浓度分别为239.95±66.37和204.89±65.12fmol/mg DNA,两组比较无差异(P>0.05)。血清睾酮两组比较无统计学意义,并与胞液、胞核受体浓度无相关关系。
Fourteen Japanese white rabbits, seven were sham-operated (SOG) and seven were vasectomy (VG). Sixteen months later, changes in testosterone receptor and serum testosterone were examined. As a result, the kd values of the cytosolic receptors of VG and SOG were 10.21 nM and 3.42 nM, respectively, and the maximum binding capacities were 33.68 fmol / mgpro and 10.42 fmol / mg pro, respectively; the kd values of nuclear receptors were 9.36 nM and 4.81 nM, the maximum binding capacity was 440.90fmol / mg DNA and 203.66fmol / mg DNA respectively. The results of single point analysis showed that the concentrations of cytosolic receptors of VG and SOG were 26.13 ± 5.64 and 8.40 ± 1.66 fmol / mg pro, respectively, and VG was significantly higher than that of SOG (P <0.01). The concentrations of nuclear receptors were 239.95 ± 66.37 and 204.89 ± 65.12fmol / mg DNA, no difference between the two groups (P> 0.05). Serum testosterone was no significant difference between the two groups, and no correlation between cytosolic and nuclear receptor concentrations.