桑叶总RNA和mRNA的分离

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<正> 材料与方法材料:温室中栽培的桑树(Morus acidosaL.),取其生长一个月的叶片20g,用作 RNA分离的原始材料。分离总 RNA 的方法:在100ml 异氰酸胍溶液(含50g 异氰酸胍,735mg 柠檬酸钠,o.7ml 巯基乙醇和500mg sarcosil)中用一台Warren 匀浆器把桑叶打成碎片。尔后,转移到50ml X_4日立聚碳酸管中,室温下用
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