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目的建立高灵敏度的猪肉组织中沙丁胺醇、莱克多巴胺和克伦特罗同时测定的方法。方法样品匀浆好后用5%高氯酸溶液振荡提取,反相C18小柱净化浓缩,以乙腈-2mmol/L醋酸铵+0.1%甲酸水溶液为流动相进行梯度洗脱,通过液相色谱-质谱以MRM方式检测,以D3-沙丁胺醇为内标定量。结果测定3种β激动剂的方法的线性范围均为0.1～10.0μg/L,线性相关系数分别为0.9998、0.9991和0.9992,定量检出限分别为0.2μg/kg、0.3μg/kg及0.5μg/kg,回收率分别为89.9%～96.7%、84.9%～90.1%及88.4%～96.2%。结论方法简便,灵敏度好,选择性好,适合于猪肉组织样品中沙丁胺醇、莱克多巴胺及克伦特罗残留进行检测及结构确证。
Objective To establish a method for simultaneous determination of albuterol, ractopamine and clenbuterol in pork with high sensitivity. Methods The sample was homogenized and extracted with 5% perchloric acid solution. The residue was purified by reversed-phase C18 column and eluted with acetonitrile-2mmol / L ammonium acetate + 0.1% formic acid as mobile phase. The mobile phase was eluted by liquid chromatography- Mass spectrometry was detected by MRM and D3-albuterol as internal standard. Results The linear range of the three β agonists was 0.1-10.0 μg / L with linear correlation coefficients of 0.9998, 0.9991 and 0.9992, respectively. The limits of quantitation were 0.2 μg / kg, 0.3 μg / kg and 0.5 μg respectively / kg, the recoveries were 89.9% ~ 96.7%, 84.9% ~ 90.1% and 88.4% ~ 96.2%, respectively. Conclusion The method is simple, sensitive and selective. It is suitable for the detection and structural confirmation of salbutamol, ractopamine and clenbuterol residues in pork tissue samples.