论文部分内容阅读
本研究以超表达拟南芥液泡膜H~+-焦磷酸酶编码基因AVP1的转基因百脉根为材料,对其耐盐性和抗旱性进行了检测。结果显示:在200 mmol·L~(-1)NaCl下处理或自然干旱7d后,转基因植株的生长虽然受到抑制,但受抑程度明显低于野生型植株,前者叶片相对含水量比后者分别高18%和14%,净光合速率分别高20%和21%,而MDA含量则分别低35%和27%,相对质膜透性分别低28%和27%。此外,随着盐和干旱胁迫的加剧,与野生型植株相比,转基因植株体内积累了更多Na~+、K~+和Ca~(2+)。以上结果表明,AVP1基因的超表达可能提高了百脉根细胞Na~+区域化能力,既减轻了过量Na~+对细胞质的毒害作用,也提高了植株的渗透调节能力,从而增强了百脉根的耐盐性和抗旱性。
In this study, we tested the tolerance to salinity and drought in transgenic Lotus japonicus plants, which overexpress the H + -pyrophosphatase encoding gene AVP1 in Arabidopsis thaliana. The results showed that although the growth of transgenic plants was inhibited at 200 mmol·L -1 NaCl or 7 days after natural drought, the inhibition degree of transgenic plants was significantly lower than that of wild type plants. The relative water content of leaves of the former was higher than that of the latter 18% and 14% higher, and 20% and 21% higher net photosynthetic rate respectively, while the content of MDA was 35% and 27% lower respectively, while the relative plasma membrane permeability was 28% and 27% lower respectively. In addition, more Na ~ +, K ~ + and Ca ~ (2+) were accumulated in the transgenic plants compared with the wild type plants as salt and drought stress aggravated. The above results show that over-expression of AVP1 gene may increase the ability of regionalization of Na ~ + in rooting cells, which not only reduces the poisoning effect of excessive Na ~ + on the cytoplasm but also enhances the osmotic adjustment ability of plants, Root salt and drought resistance.