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[目的]探讨银杏总黄酮对非酒精性脂肪肝(NAFLD)大鼠发病过程中生化指标及血清脂联素(Adipo)水平的影响。[方法]72只SPF级雄性大鼠随机分为3组:正常对照组、NAFLD模型组、银杏总黄酮治疗组。利用高脂饲料喂养法构建NAFLD大鼠模型。观察各组大鼠肝脏组织形态改变。全自动生化分析仪测定血清丙氨酸转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、三酰甘油(TG)、总胆固醇(TC),酶联免疫吸附法(ELISA)测定血清Adipo含量,RT-PCR和Western-blot法分别测定肝组织AdipoR_2mRNA及蛋白表达。[结果]随着时间延长,NAFLD模型组大鼠血清ALT、AST、TG、TC含量逐渐增高,血清Adipo含量逐渐减少,肝组织中AdipoR_2mRNA及蛋白表达水平逐渐下降;银杏总黄酮治疗组大鼠血清ALT、AST、TG、TC含量逐渐降低,血清Adipo含量、肝组织AdipoR_2mRNA及蛋白表达水平逐渐增加,与NAFLD组比较均差异有统计学意义(P<0.05)。[结论]银杏总黄酮对NAFLD大鼠肝脏有防治作用,降低血清ALT、AST、TG、TC含量,可能通过增加AdipoR_2表达所致。
[Objective] To investigate the effects of ginkgo flavonoids on biochemical indexes and serum Adipo levels in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) in rats. [Methods] Seventy-two SPF male rats were randomly divided into three groups: normal control group, NAFLD model group and ginkgo total flavonoid treatment group. NAFLD rat model was constructed by high fat diet feeding. The morphological changes of liver in each group were observed. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC) and serum Adipo The mRNA and protein expression of AdipoR_2 in liver tissue were determined by RT-PCR and Western-blot respectively. [Result] With the prolongation of time, the levels of ALT, AST, TG and TC in the serum of NAFLD model rats gradually increased, the content of Adipo decreased gradually and the expression level of AdipoR_2 mRNA and protein in hepatic tissue decreased gradually. The levels of serum The content of ALT, AST, TG and TC decreased gradually. The content of Adipo and the expression of AdipoR 2 mRNA and protein in liver increased gradually. Compared with NAFLD group, there was significant difference (P <0.05). [Conclusion] The total flavonoids of Ginkgo biloba could prevent and treat the liver of NAFLD rats and decrease the content of ALT, AST, TG and TC in serum, which may be caused by increasing the expression of AdipoR_2.