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目的:研究苯妥英钠对慢性应激大鼠海马CA3区锥体神经元形态结构的影响。方法:采用Nissl染色,Golgi染色及电子显微镜技术观察各组大鼠海马CA3区锥体神经元的形态结构。结果:慢性应激引起了海马CA3区神经元的丢失(由39±4减少至35±4)、顶树突总长度的缩短(由196μm±35μm缩短至156μm±33μm,P<0.05),以及神经元超微结构的变性。苯妥英钠显著抑制了慢性应激所致海马CA3区神经元的减少(38.4±2.2)及顶树突总长度的缩短(198μm±36μm,P<0.05),改善了神经元超微结构的变性。结论:慢性应激可导致大鼠海马CA3区锥体神经元损伤,苯妥英钠对应激状态下的海马CA3区锥体神经元具有保护作用。
Objective: To investigate the effect of phenytoin sodium on the morphology and structure of pyramidal neurons in CA3 hippocampus of chronic stress rats. Methods: Nissl staining, Golgi staining and electron microscopy were used to observe the morphological changes of hippocampal CA3 pyramidal neurons in each group. RESULTS: Chronic stress caused a loss of neurons in the hippocampal CA3 region (from 39 ± 4 to 35 ± 4), shortening of the total dendrites (from 196 μm ± 35 μm to 156 μm ± 33 μm, P <0.05), and Degeneration of neuronal ultrastructure. Phenytoin significantly inhibited the decrease of neurons in hippocampal CA3 region (38.4 ± 2.2) and the shortening of total dendrites (198μm ± 36μm, P <0.05) induced by chronic stress, and improved the degeneration of neuronal ultrastructure. CONCLUSION: Chronic stress can induce pyramidal neuronal damage in hippocampal CA3 region of rats, and phenytoin sodium has protective effect on hippocampal CA3 pyramidal neurons under stress.