论文部分内容阅读
目的:探讨Occludin mRNA、蛋白表达水平与胃癌发生发展、侵袭转移的关系。方法:应用组织芯片免疫组化染色和实时荧光定量PCR的方法,检测occludin在胃腺癌组织中的表达并分析其与临床特征的相关性。结果:免疫组织化学检测显示,Occludin在癌旁正常胃黏膜上皮中阳性表达率高于胃腺癌组织(96.9%vs85.4%,84.6%,89.2%,78.5%)(P<0.05);Occludin的表达水平与胃腺癌TNM分期和分化程度有关(P<0.05)。实时荧光定量PCR的检测结果显示,Occludin mRNA在胃癌组织和癌旁正常胃组织中的表达无明显差异(P>0.05)。结论:Occludin蛋白的表达水平与胃腺癌的发生发展和侵袭转移呈负相关。
Objective: To investigate the relationship between Occludin mRNA and protein expression and the development, invasion and metastasis of gastric cancer. Methods: The expression of occludin in gastric adenocarcinoma was detected by immunohistochemistry and real-time fluorescence quantitative PCR, and its correlation with clinical features was analyzed. Results: The positive rate of Occludin in gastric mucosal epithelium was significantly higher than that in gastric adenocarcinoma (96.9% vs 85.4%, 84.6%, 89.2%, 78.5%) (P <0.05) by immunohistochemistry. The expression level was related to TNM staging and differentiation of gastric adenocarcinoma (P <0.05). The results of real-time quantitative PCR showed that the expression of Occludin mRNA in gastric cancer tissues and adjacent normal gastric tissues showed no significant difference (P> 0.05). Conclusion: The expression level of Occludin protein is negatively correlated with the occurrence, development and invasion and metastasis of gastric adenocarcinoma.