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目的建立黔产辣蓼药材的定性、定量检测方法,并制定质量标准。方法对黔产辣蓼药材进行性状鉴别、显微鉴别,采用薄层色谱(TLC)法对其中的槲皮素进行定性鉴别,采用高效液相色谱(HPLC)法对其中的槲皮苷进行含量测定。按照《中华人民共和国药典》2015版(一部)附录方法对辣蓼药材中的水分、总灰分、酸不溶性灰分、浸出物进行测定。结果薄层色谱斑点清晰,无干扰,专属性强;含量测定槲皮苷在0.462~4.158μg范围内线性关系良好,Y=79.004X+0.520,r=0.999 8,平均回收率为98.00%;初步拟定水分不得超过14.0%,总灰分不得超过14.0%,酸不溶性灰分不得超过5.0%,浸出物不得超过10.0%。结论本方法操作简单,结果可靠,可有效控制辣蓼药材的质量。
Objective To establish a qualitative and quantitative method for the determination of Polygonum capus in Guizhou and to set the quality standard. Methods Polygonum caprifoliacens from Guizhou were identified and microscopically characterized by TLC. The contents of quercetin in them were determined by high performance liquid chromatography (HPLC) Determination. According to “The People’s Republic of China Pharmacopoeia” 2015 edition (a) appendix method of Polygonum hydropiper herbs moisture, total ash, acid insoluble ash, extracts were determined. Results The chromatographic spots were clear, non-interfering and specific. The content of quercetin in the range of 0.462 ~ 4.158μg was good, Y = 79.004X + 0.520, r = 0.999 8, the average recovery was 98.00% The proposed moisture content shall not exceed 14.0%, the total ash content shall not exceed 14.0%, the acid-insoluble ash content shall not exceed 5.0% and the extract content shall not exceed 10.0%. Conclusion This method is simple, reliable, and can effectively control the quality of Polygonum.