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目的:本实验通过观察越婢加半夏汤对过敏性哮喘小鼠血清中免疫球蛋白E(Ig E)和肺组织匀浆中白介素4(IL-4)的影响,探讨越婢加半夏汤调节过敏性哮喘肺组织病理及免疫功能紊乱的机制。方法:健康昆明小鼠40只随机分为正常对照组、哮喘模型组、西医组、中医组,每组各10只,哮喘模型组、西医组、中医组分别采用卵白蛋白(OVA)、氢氧化铝凝胶混悬液腹腔注射致敏、雾化激发等方法建立小鼠哮喘模型,后分别用生理盐水、地塞米松、越婢加半夏汤对哮喘小鼠进行干预。治疗完成后处死各组小鼠,肺组织HE染色,并采用ELISA法检测小鼠血清Ig E及肺组织匀浆中IL-4水平,观察其病理变化。结果:中医组小鼠与哮喘模型组小鼠相比,前者血清中Ig E含量下降(P<0.05);前者肺组织肺组织匀浆中IL-4明显低于后者(P<0.05)。结论:降低过敏性哮喘小鼠血清中Ig E含量及肺组织匀浆中IL-4浓度,是越婢加半夏汤治疗过敏性哮喘的可能作用机制之一。
Objective: The purpose of this experiment is to observe the effect of Xietong plus Banxia Decoction on serum IgE and IL-4 in the lung homogenate of allergic asthma mice, Soup Regulates the Pathology and Immune Function of Allergic Asthma Pulmonary Tissue. Methods: Forty Kunming mice were randomly divided into normal control group, asthma model group, western medicine group and TCM group, 10 in each group. Asthma model group, western medicine group and TCM group were treated with ovalbumin (OVA) The mice model of asthma was established by intraperitoneal injection of aluminum gel suspension and sensitization by atomization, and then the asthmatic mice were intervened by normal saline, dexamethasone and Yuexuan plus Banxia Decoction respectively. The mice in each group were sacrificed after treatment. The lung tissues were stained with HE. The levels of Ig E in serum and IL-4 in lung homogenates were detected by ELISA. The pathological changes were observed. Results: Compared with the asthma model group, the content of IgE in the serum of the Chinese medicine group decreased (P <0.05). The former IL-4 level in the lung tissue homogenate of the former was lower than the latter (P <0.05). CONCLUSIONS: It is one of the possible mechanisms of the treatment of Allergic asthma by Xuanji plus Banxia Decoction to reduce Ig E level in serum and IL-4 concentration in lung homogenate of mice with allergic asthma.