论文部分内容阅读
目的:探讨美洲大蠊多肽对裸鼠人肝细胞Bel-7402移植瘤生长及血管生成的抑制作用。方法:建立裸鼠人肝癌细胞株Bel-7402移植瘤模型,观察美洲大蠊多肽对肿瘤生长、微血管密度(MVD)和血管内皮生长因子(VEGF)表达的影响。结果:美洲大蠊多肽不同程度抑制裸鼠移植瘤的生长,各治疗组给药后肿瘤体积、相对肿瘤体积和相对肿瘤增殖率明显下降,与对照组比较,P<0.05;美洲大蠊多肽治疗组微血管分布较稀疏,MVD值由对照组的(64.77±13.49)分别降低到(41.52±5.36),(35.56±5.12)和(30.28±4.17),差异有统计学意义(P<0.05或P<0.01)。美洲大蠊多肽治疗组能明显减少VEGF的阳性表达,阳性表达率由对照组的(75.35±13.89)%减少到(59.77±12.07)%,(42.19±11.57)%和(35.88±10.26)%,差异有统计学意义(P<0.05或P<0.01)。结论:美洲大蠊多肽能明显抑制Bel-7402裸鼠移植瘤的生长,降低肿瘤MVD,减少瘤组织中VEGF的表达,揭示其抗肿瘤作用与抑制血管生成有关。
OBJECTIVE: To investigate the inhibitory effect of the American cockroach peptide on the growth and angiogenesis of human hepatocellular Bel-7402 xenografts in nude mice. Methods: The nude mice model of human hepatocellular carcinoma cell line Bel-7402 was established to observe the effect of the polypeptide of Periplaneta americana on tumor growth, microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression. Results: The American cockroach peptide could inhibit the growth of xenografts in nude mice to varying degrees. The volume of tumor, the relative tumor volume and the relative tumor proliferation rate decreased significantly in each treatment group compared with the control group (P <0.05) The microvessel distribution was sparse and the MVD decreased from (64.77 ± 13.49) to (41.52 ± 5.36), (35.56 ± 5.12) and (30.28 ± 4.17) in the control group (P <0.05 or P < 0.01). Compared with control group, the expression of VEGF was significantly decreased in the treatment group of American cockroach peptide (59.77 ± 12.07%), (42.19 ± 11.57)% and (35.88 ± 10.26)%, respectively, from 75.35 ± 13.89% The difference was statistically significant (P <0.05 or P <0.01). Conclusion: The American cockroach peptide can significantly inhibit the growth of Bel-7402 xenografts in nude mice, reduce the MVD and reduce the expression of VEGF in tumor tissue, revealing that its anti-tumor effect is related to inhibiting angiogenesis.