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目的探讨影像学检查在骨髓间质干细胞(mesenchymal stem cells,MSCs)治疗缺血性脑卒中实验研究中的应用价值。方法雄性成年SD大鼠,用随机数字表法分假手术组(Sham组,n=5)、脑缺血组再灌注组(MCAO组,n=25)、PBS治疗组(n=25)和MSCs治疗组(n=25),每组分别于造模成功后1、3、7、14、28 d行CT灌注成像(CT perfusionimaging,CTPI)、MRI检查、神经功能评分、2,3,5-三苯基氯化四氮唑(TTC)染色、HE染色及微血管密度分析。结果Sham组每时间点血流动力学未见明显异常,脑组织未见明显梗死区域。MCAO组、PBS组、MSCs组:①CTPI参数图、T2WI图与TTC染色显示梗死体积差异无统计学意义;MSCs组与MCAO组、PBS组比较,7、14、28 d梗死体积差异有统计学意义(P<0.05)。②MSCs组与MCAO组、PBS组比较,rCBF在7、14、28 d升高,差异有统计学意义(P<0.05);神经功能评分减低明显,微血管密度明显增高,HE染色示缺血周边区细胞变性坏死程度减低。MSCs组3 d及7 d在梗死灶周围见点状长T1、短T2信号,14 d及28 d低信号向梗死病灶方向弥散,强度减低。结论CTPI和MRI影像技术可以从形态学及血流动力学方面观察MSCs治疗缺血性脑卒中的疗效,磁标记MSCs的MRI检查实现了活体追踪标记细胞在脑内的存活、迁移及分化。
Objective To investigate the value of imaging examination in the experimental study of treating mesenchymal stem cells (MSCs) with ischemic stroke. Methods Male adult Sprague-Dawley rats were randomly divided into sham operation group (Sham group, n = 5), cerebral ischemia reperfusion group (MCAO group, n = 25) and PBS group MSCs group (n = 25). CT perfusion imaging (CTPI), MRI examination, neurological function score, 2, 3, 5 Triphenyltetrazolium chloride (TTC) staining, HE staining and microvessel density analysis. Results Sham group at each time point hemodynamics no obvious abnormalities, no obvious brain infarction area. MCAO group, PBS group and MSCs group: (1) There was no significant difference in infarct volume between CTPI parameter map, T2WI map and TTC staining; infarction volume of MSCs group was significantly different from MCAO group and PBS group (P <0.05). (2) Compared with MCAO group and PBS group, the rCBF increased at 7, 14 and 28 days in MSCs group (P <0.05), the neurological score decreased significantly and the microvessel density increased obviously. Reduce the degree of cell degeneration and necrosis. On the 3rd and 7th day of MSCs, punctate long T1 and short T2 signals were seen around the infarct, and low signal on the 14th and 28th day diffused toward the infarct and the intensity was decreased. Conclusion CTPI and MRI can observe the therapeutic effect of MSCs on ischemic stroke morphologically and hemodynamically. MRI examination of magnetic labeled MSCs realizes the survival, migration and differentiation of labeled cells in the brain.