论文部分内容阅读
【目的】明确几种不同配方对牛粪、羊粪、鸡粪有机肥发酵过程中菌群变化的影响及其育苗效果。【方法】以不同配方分别处理牛、羊、鸡粪,经30 d的保温、厌氧发酵制成有机肥,采用PCR-DGGE、Biolog生态板技术解析菌群变化情况及碳代谢活性;采用番茄穴盘育苗实验评价肥效。【结果】从DGGE分离菌肥微生物结果为乳酸杆菌属(Lactobacillus sp.)和魏斯氏菌属(Weissella sp.)所占比例较大,乳酸杆菌属(Lactobacillus sp.)为40.4%魏斯氏菌属(Weissella sp.)为23.8%;从平均颜色变化率(AWCD)可以看出Shannon-Wiener指数(H’)羊粪要高于鸡粪和牛粪的水平;主成分分析显示牛粪组在3个处理的菌群碳源利用水平均存在差异;羊粪组Y1和Y2菌群群落结构相对较一致,处理Y3和Y4存在差异;鸡粪组处理J2和J3群落结构相对较一致,处理J1和J4则存在差异。不同的处理方法对植株的4个指标影响各不相同,存在较大差异性。【结论】不同配方处理对牛、羊、鸡粪有机肥菌群结构造成显著影响,N3、Y3和J1处理的肥效最佳,番茄苗株高平均比对照高出25.5%、22.03%和26.16%。
【Objective】 The purpose of this study is to clarify the effect of several different formulas on the changes of bacterial population during the fermentation of cow manure, sheep manure and chicken manure and the effect of raising seedlings. 【Method】 The bovine, sheep and chicken manure were treated with different formulations respectively. After 30 days of incubation, the anaerobic fermentation was used to make organic fertilizer. The changes of bacterial population and carbon metabolic activity were analyzed by PCR-DGGE and Biolog. Plug culture experiment to evaluate fertilizer efficiency. 【Result】 The results showed that the microorganisms from DGGE had a large proportion of Lactobacillus sp. And Weissella sp., Lactobacillus sp. 40.4% Weiss Weissella sp. Was 23.8%. From the average color change rate (AWCD), the Shannon-Wiener index (H ’) sheep manure was higher than that of chicken manure and cow manure. The principal component analysis showed that cow manure group The utilization of carbon sources in the three treatment groups was different; the structure of Y1 and Y2 in sheep manure group was relatively consistent and Y3 and Y4 were different; the structure of J2 and J3 in chicken manure group was relatively consistent, and the treatment of J1 And J4 are different. Different treatment methods have different effects on the four indicators of plants, there is a big difference. 【Conclusion】 The effects of different formula treatments on the structure of organic manure of cattle, sheep and chicken manure were obvious. N3, Y3 and J1 treatments had the best fertilizer efficiency. The average height of tomato seedlings was 25.5%, 22.03% and 26.16% .