目的建立反相高效液相色谱法测定清开灵颗粒剂中绿原酸含量。方法AtlantisTMdC18色谱柱(4.6×150mm,5μm);流动相为甲醇-水-冰醋酸(15:85:0.5),流速1ml/min,检测波长327nm,10μL进样环。结果绿原酸线性范围为12.5～200μg/ml,平均回收率分别为101.7%,RSD为1.07%。结论本法操作简便、结果准确,可以用作清开灵颗粒剂的质量控制。 Objective To establish a RP-HPLC method for determination of chlorogenic acid in Qingkailing granules. Methods The AtlantisTM dC18 column (4.6×150mm, 5μm) was used. The mobile phase was methanol-water-glacial acetic acid (15:85:0.5), the flow rate was 1ml/min, the detection wavelength was 327nm, and the 10μL injection loop was used. Results The linear range of chlorogenic acid was 12.5-200 μg/ml. The average recovery was 101.7% and the RSD was 1.07%. Conclusion This method is simple and accurate, and can be used as a quality control agent for Qingkailing granules.