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目的:研究卵巢上皮性肿瘤中nm23H1基因遗传不稳定性与卵巢肿瘤进展的关系,为揭示nm23H1基因作用机制和肿瘤转移机制提供实验依据。方法:采用石蜡包埋组织抽提DNA,PCR-单链构象多态性(PCR-SSCP),常规银染、Envision免疫组织化学染色和Leica-Qwin计算机图像分析等方法,对石蜡包埋的卵巢上皮性肿瘤及相应的正常组织,进行17号染色体D17S396位点微卫星不稳定性、杂合性缺失的检测和nm23H1基因的表达研究。结果:本实验中,卵巢上皮性癌D17S396位点遗传不稳定发生率为40%,明显高于交界性肿瘤的10%,而在良性肿瘤和正常卵巢组织中未见该位点遗传不稳定的发生。结论:nm23H1基因的遗传不稳定性可能是卵巢上皮性癌发生、发展的一个重要因素,杂合性缺失的发生可作为卵巢组织恶变的判断指标之一。
OBJECTIVE: To study the relationship between the genetic instability of nm23H1 gene and the progression of ovarian tumors in ovarian epithelial neoplasia, and provide experimental evidence for revealing the mechanism of action of nm23H1 gene and tumor metastasis mechanism. Methods: Paraffin-embedded ovaries were excised by DNA extraction, PCR-SSCP, silver staining, Envision immunohistochemistry and Leica-Qwin computer image analysis. Epithelial tumors and corresponding normal tissues, microsatellite instability at chromosome 17 D17S396, loss of heterozygosity and expression of nm23H1 gene were studied. Results: In this study, the incidence of genetic instability at D17S396 locus in ovarian epithelial carcinoma was 40%, which was significantly higher than that in borderline tumors (10%). However, no genetic instability was observed in benign tumors and normal ovarian tissues occur. Conclusion: The genetic instability of nm23H1 gene may be an important factor for the occurrence and development of epithelial ovarian cancer. The occurrence of heterozygosity may be one of the indicators of ovarian malignancy.