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标本由开罗传染病生物医学研究中心,在郊区2岁以下婴儿中采得。11份腹泻病例和10份对照便样。腹泻病例如下:三例鲍氏志贺菌,四例福氏志贺菌、四例宋内氏志贺菌。将含水便样离心,用有机溶剂萃取,并衍生成羟酸,醇、羟茎酸和胺的特异性电子捕获衍生物。在一台带有电子捕获鉴定器及高分辨的玻璃柱的气相色谱上进行分析。得到以FPEC—GLC谱图具有不同菌的代谢产物的标志。通过这些标志,区别以上几种志贺氏菌和产生耐热性或不耐热肠毒素大肠埃希氏杆菌。
Specimens were collected by the Biomedical Research Center for Infectious Diseases, Cairo, in infants under the age of 2 in the suburbs. 11 diarrhea cases and 10 control samples. Diarrhea cases are as follows: three cases of Shigella sonnei, four cases of Shigella flexneri, four cases of Shigella sonnei. The hydrous samples are centrifuged, extracted with organic solvents, and derivatized to specific electron capture derivatives of hydroxy acids, alcohols, hydroxytoluene and amines. Analyze on a gas chromatograph with electron capture detector and high resolution glass column. The metabolites with different bacteria in the FPEC-GLC spectrum were obtained. Through these markers, the above several kinds of Shigella and produce heat-resistant or heat-labile enterotoxigenic Escherichia coli.