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目的:建立以RP-HPLC法测定资木瓜中原儿茶酸含量的方法。方法:色谱柱:Agilent Eclipse XDB-C_(18)(250 mm×4.6mm,5μm),流动相:乙腈-0.1%磷酸水溶液(5:95),流速1 ml·min~(-1),检测波长260 nm。结果:原儿茶酸进样量在0.02~0.20μg范围内具有良好的线性关系(r=0.9999),平均加样回收率为101.23%,RSD为1.83%(n=5)。结论:本方法简便、准确、重复性好,可用于控制资木瓜的质量。
Objective: To establish a method for the determination of protocatechuic acid in papaya leaves by RP-HPLC. METHODS: The column was Agilent Eclipse XDB-C 18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution (5:95) and the flow rate was 1 ml · min -1 Wavelength 260 nm. Results: The calibration curve of protocatechuic acid was linear in the range of 0.02-0.20 μg (r = 0.9999). The average recovery was 101.23% and the RSD was 1.83% (n = 5). Conclusion: The method is simple, accurate, reproducible and can be used to control the quality of papaya.