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本文报告了抗血吸虫药硝硫氰胺(7505)的放射免疫测定法。以7505与卵清白蛋白或猪IgG结合作抗原以免疫家兔;~(131)I标记BSA-7505作标记抗原;并采用聚乙二醇(PEG)为分离剂。测定范围为0.64～400 ng,重复性良好。PEG的分离效果与双抗体法一致。4只家兔经一次胃饲7505(120 mg/kg体重)后,各兔血中7505浓度的变化趋势基本一致。给药后第2～3天时血中7505浓度达高峰,结果与胃饲~3H-7505测定~3H放射性的类似。同时并对14种7505的衍化物作了放射免疫交叉反应试验,试图探索其与结构及实验性疗效的关系。结果表明,交叉反应出现于具有基本结构的各化合物,倘改变两苯环间的则与7505的抗血清无交叉反应。交叉反应与药效的初筛结果不同,后者当两苯环间的改为-O-并不受到影响。根据交叉反应的结构特征,7505抗血清同样可应用于若干7505衍化物的定量测定。
This article reports the radioimmunoassay of the anti-schistosome drug, nitrofurazone (7505). Rabbit was immunized with 7505 antigen combined with ovalbumin or porcine IgG; ~ (131) I labeled BSA-7505 was used as labeled antigen; and polyethylene glycol (PEG) was used as separating agent. The measurement range of 0.64 ~ 400 ng, good reproducibility. The separation of PEG is consistent with the double antibody method. After a single stomach fed with 7505 (120 mg / kg body weight) in 4 rabbits, the trend of 7505 concentration in each rabbit was basically the same. After the administration of the first 2 to 3 days when the peak concentration of 7505 in blood, the results and ~ 3H-7505 stomach Determination of ~ 3H radioactive similar. At the same time, 14 kinds of derivatives of 7505 were tested by radioimmunoassay in order to explore their relationship with the structure and experimental efficacy. The results show that the cross-reaction occurs in the basic structure of the compounds, if the change between the two benzene rings and 7505 antisera no cross-reaction. Cross-reaction and efficacy of the screening results are different, the latter when the two benzene ring to -O- is not affected. Based on the structural characteristics of the cross-reaction, the 7505 antiserum is equally applicable to the quantitative determination of several 7505 derivatives.