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目的:研究白藜芦醇(resveratrol,Res)对中波紫外线(UVB)照射体外培养的人永生化角质形成细胞(HaCaT)的损伤的保护作用。方法:体外培养HaCaT细胞,MTT法检测不同浓度Res对HaCaT细胞增殖活性的影响。UVB照射及Res处理24 h后,采用RT-PCR方法测定细胞中MMP-1,MMP-9和TIMP-1mRNA的相对含量。结果:UVB 30 mJ.cm-2照射后,HaCaT细胞产生的MMP-9 mRNA含量显著增加(P<0.05),TIMP-1 mRNA明显减少(P<0.05);UVB 90 mJ.cm-2照射HaCaT细胞后其MMP-1 mRNA增加(P<0.05)。与UVB照射组相比较,0.5 mmol.L-1Res能显著抑制UVB诱导的HaCaT细胞产生的MMP-1,MMP-9 mRNA含量增加以及UVB对TIMP-1 mRNA的抑制作用(P<0.05)。结论:Res可以显著抑制UVB照射诱导的HaCaT细胞产生的MMP-1,MMP-9及TIMP-1 mRNA含量的变化,对皮肤光老化可有一定的防护作用。
Objective: To study the protective effect of resveratrol (Res) on human immortalized keratinocytes (HaCaT) induced by UVB irradiation. Methods: HaCaT cells were cultured in vitro. The effects of different concentrations of Res on the proliferation of HaCaT cells were detected by MTT assay. After UVB irradiation and Res treatment for 24 h, the relative content of MMP-1, MMP-9 and TIMP-1 mRNA in the cells was determined by RT-PCR. Results: After exposure to UVB 30 mJ · cm-2, the mRNA expression of MMP-9 in HaCaT cells was significantly increased (P <0.05) and TIMP-1 mRNA was significantly decreased (P <0.05) MMP-1 mRNA increased (P <0.05). Compared with UVB group, 0.5 mmol.L-1Res significantly inhibited UVB-induced increase of MMP-1 and MMP-9 mRNA expression in HaCaT cells and UVB inhibited TIMP-1 mRNA expression (P <0.05). CONCLUSION: Res can significantly inhibit the changes of MMP-1, MMP-9 and TIMP-1 mRNA induced by UVB in HaCaT cells, which may have a protective effect on skin photoaging.