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目的:建立测定夜宁糖浆中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的方法。方法:采用高效液相色谱法,色谱条件为Agilent HC C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-0.018mol.L-1醋酸(21∶79),流速0.8mL.min-1,柱温30℃,检测波长320nm。结果:2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.016~0.320g.L-1范围内有良好的线性关系(r=0.999 9),平均加样回收率为98.5%,RSD为1.37%。结论:本方法准确,重复性好,可用于夜宁糖浆中2,3,5,4’-羟基二苯乙烯-2-O-β-D-葡萄糖苷的测定。
Objective: To establish a method for the determination of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside in nying ning syrup. Methods: The HPLC conditions were Agilent HC C18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile-0.018 mol·L-1 acetic acid (21:79) at a flow rate of 0.8 mL · min- 1, column temperature 30 ℃, detection wavelength 320nm. Results: There was a good linear relationship between 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and 0.016-0.320gL-1 (r = 0.999 9) The sample recovery was 98.5% with a RSD of 1.37%. Conclusion: The method is accurate and reproducible. It can be used for the determination of 2,3,5,4’-hydroxy-stilbene-2-O-β-D-glucoside in Yining decoction.