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为进一步探讨运动心脏重塑的发生机制 ,本研究采用反转录聚合酶链式反应 (RT -PCR)技术 ,对 60只SD大鼠 1 2周耐力训练过程中心肌胰岛素样生长因子 (IGF -Ⅰ )的表达进行了观察。结果表明 ,与安静对照组相比 ,耐力训练过程中心肌组织中IGF -Ⅰ在转录水平的表达均显著增强 ,其中 ,心房IGF-ⅠmRNA的表达在训练 1 0天达到高峰 (P <0 0 1 ) ,心室IGF -ⅠmRNA的表达在训练 3天即达到高峰 (P <0 0 1 ) ,训练 40天后心房、心室IGF -ⅠmRNA的表达基本恢复到正常对照水平。结果提示 ,心血管调节肽IGF -Ⅰ在运动性心脏重塑发生过程中起上调作用 ,且这种上调作用在运动心脏发生的早期即已启动 ,心房和心室组织中IGF -Ⅰ的调节作用在时间和方式上存有差异
In order to further explore the mechanism of cardiac remodeling, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of insulin-like growth factor (IGF- Ⅰ) expression was observed. The results showed that compared with the quiet control group, the expression of IGF-Ⅰ in myocardial tissue during endurance training increased significantly, and the expression of atrial IGF-ⅠmRNA peaked at day 10 (P <0.01) ), The expression of IGF-Ⅰ mRNA in the ventricle peaked 3 days after training (P <0.01). After training for 40 days, the expression of IGF-ⅠmRNA in the atria and ventricle returned to the normal level. The results suggest that the cardiovascular regulatory peptide IGF-I plays an up-regulation role in the process of exercise-induced cardiac remodeling and this up-regulation is initiated early in the development of exercise-induced heart and the regulation of IGF-I in atrial and ventricular tissues is at There are differences in time and mode