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目的以体外培养的大鼠视网膜Müller细胞的变化入手探讨糖尿病性视网膜病变(DR)新生血管的成因。方法原代培养的大鼠视网膜Müller细胞在不同葡萄糖浓度的培养液中培养,培养72 h后,MTT法检测细胞增殖,利用Protein Pathway Array(PPA)技术检测细胞内146磷酸化和非磷酸化蛋白质的表达情况。结果 Müller细胞的增长呈葡萄糖浓度依赖性(50 mmol/L葡萄糖浓度是增殖最强)。50 mmol/L高糖条件下,在视网膜Müller细胞中检测出47种磷酸化和非磷酸化差异表达蛋白质(如XIAP,VEGF,HIF1α,NF-κB等),这些蛋白质涉及细胞生存、增殖、凋亡等几个重要信号传导通路。结论高糖能够刺激视网膜Müller细胞增殖,这种增殖作用是Müller细胞中多条重要信号传导通路共同参与的结果。
OBJECTIVE To investigate the genesis of neovascularization in diabetic retinopathy (DR) based on the changes of rat retinal Müller cells in vitro. Methods Primary cultured rat retinal Müller cells were cultured in culture medium with different glucose concentrations. After cultured for 72 h, MTT assay was used to detect the proliferation of rat retinal Müller cells. The protein levels of 146 phosphorylated and non-phosphorylated proteins The expression of the situation. Results Müller cells showed glucose concentration-dependent growth (50 mmol / L glucose was the most proliferative). Forty-seven phosphorylated and nonphosphorylated differentially expressed proteins (such as XIAP, VEGF, HIF1α, NF-κB, etc.) were detected in retinal Müller cells under 50 mmol / L high glucose conditions. These proteins involved in cell survival, proliferation, Death and several other important signal transduction pathway. Conclusion High glucose can stimulate the proliferation of retinal Müller cells. This proliferation is the result of multiple important signal transduction pathways in Müller cells.