本文报道应用含血清培养液及无血清培养液进行细胞融合,共获得8株稳定分泌抗纤维连接蛋白单克隆抗体杂交瘤细胞株。Western Blot证实McAb仅识别220KD的Fn。角膜冰冻切片,McAb经PAP法显色,Fn分布带与文献报道相同。ELISA双抗体夹心法测定18例献血员血浆Fn含量,结果为0.342±0.026mg/ml,与文献报道极为相近,且重复性好。 This article reports the use of serum-containing culture medium and serum-free medium for cell fusion, a total of eight stable monoclonal antibody secreting anti-fibronectin hybridoma cell lines. Western Blot confirmed that McAb recognizes only FK of 220KD. Frozen sections of cornea, McAb color by PAP method, Fn distribution with the same reported in the literature. ELISA double antibody sandwich method for the determination of 18 blood donors plasma Fn content, the result was 0.342 ± 0.026mg / ml, reported very closely with the literature, and good reproducibility.