论文部分内容阅读
迄今,慢性渗出性中耳炎的粘膜活检主要注重研究其粘液质(mucosubstances),本文力图揭示分泌性中耳炎的病程与上皮,粘液质特征的关系,以及粘膜下组织的细胞型式。作者们对16例 (19耳)初次或多次鼓膜切开放置通气管的患者(病程6月至数年)进行中耳粘膜活检,标本固定包埋后作7μm的连续切片,用苏木精-伊红、过碘酸雪夫试剂(PAS)及阿辛蓝(alcian blue)染色后检查,其中7个耳标本以神经氨酸酶处理后重染色。结果:初次安置通气管的病例,粘膜上皮阿辛蓝及过碘酸雪夫染色阳性,围绕标本的渗出液多被阿辛蓝深染,粘膜上皮通常为2～3层细胞,有的可见纤毛,纤毛细胞偶由单层扁平上皮
To date, mucosal biopsies of chronic exudative otitis media have focused primarily on their mucosubstances. This paper attempts to elucidate the relationship between the duration of secretory otitis media and epithelial and mucinous features, as well as the cell types of submucosal tissues. The authors performed 16 middle ear mucosal biopsies of 16 patients (19 ears) who underwent first or multiple tympanotomy for placement of the ventilation tube (duration of 6 months to several years). The specimens were fixed and embedded for 7 μm serial sections and stained with hematoxylin - Eosin, periodic acid Schiff ’s reagent (PAS) and alcian blue staining, 7 of which were re-stained with neuraminidase. Results: The first placement of ventilation tube cases, mucosal epithelial Asin blue and periodic acid Schiff staining positive, around the exudate specimens mostly Asin blue stained mucosal epithelium is usually 2 to 3 layers of cells, some visible cilia Ciliated cells even by a single flat epithelium