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目的通过研究双氢青蒿素(dihydroartemisinin,DHA)对狼疮模型MRL/lpr小鼠肾脏病变和肾皮质Fractalkine(FKN)、NF-κB表达的作用,并与泼尼松的作用进行比较,以探讨青蒿素治疗狼疮性肾炎的可能作用机制,试图明了双氢青蒿素对狼疮性肾炎FKN表达的调节作用。方法选取32只12周龄的雌性MRL/lpr小鼠,随机分为4组:DHA组,每天给予DHA灌胃;泼尼松组,每天给予泼尼松(prednisone,P)灌胃;DHA+泼尼松组,每天给予DHA和泼尼松灌胃;正常对照组,每天给予生理盐水灌胃;共8周。通过光镜观察肾组织病理改变,通过RT-PCR检测肾皮质FKN和NF-κB p65 mRNA的表达,免疫组织化学染色法检测肾皮质FKN及NF-κB p65蛋白的表达。结果 MRL/lpr小鼠肾皮质中检测到FKN、NF-κB p65 mRNA和蛋白的表达,DHA可减少MRL/lpr小鼠的蛋白尿、改善肾功能、减轻肾脏病变。DHA下调MRL/lpr小鼠肾皮质中FKN、NF-κB p65 mRNA和蛋白的表达。结论双氢青蒿素可通过调节FKN和NF-κB的表达实现其对狼疮性肾炎的治疗作用,与泼尼松联用效果更佳。
Objective To investigate the effect of dihydroartemisinin (DHA) on renal lesions and expression of Fractalkine (FKN) and NF-κB in lupus model MRL / lpr mice and to compare with the effect of prednisone The possible mechanism of artemisinin in the treatment of lupus nephritis attempts to clarify the regulation of dihydroartemisinin on the expression of FKN in lupus nephritis. Methods Thirty-two female MRL / lpr mice of 12 weeks old were randomly divided into 4 groups: DHA group, DHA group, DHA group, prednisone group, prednisone group, Nisong group was given daily DHA and prednisone gavage; normal control group, given daily saline; a total of 8 weeks. The pathological changes of renal tissues were observed by light microscope. The expressions of FKN and NF-κB p65 mRNA in renal cortex were detected by RT-PCR. The expressions of FKN and NF-κB p65 protein in renal cortex were detected by immunohistochemical staining. Results The expression of FKN and NF-κB p65 mRNA and protein were detected in the renal cortex of MRL / lpr mice. DHA reduced the proteinuria of MRL / lpr mice, improved renal function and decreased renal pathology. DHA down-regulated the expression of FKN, NF-κB p65 mRNA and protein in renal cortex of MRL / lpr mice. Conclusions Dihydroartemisinin can achieve its therapeutic effect on lupus nephritis by regulating the expression of FKN and NF-κB, which is better than prednisone.