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目前快速诊断肺结核仍然主要依赖光学显微镜检测痰中耐酸杆菌,然而仅1/2～3/4的病例痰涂片为阳性。从外周血细胞免疫激活证据表明,结核菌通常可进入其致敏效应细胞的血流中。现报道聚合酶链反应(PCR)检测可疑结核病患者血液标本的结果。收集血液的对象为:①痰涂片证实为活动性肺结核8例,均未使用过抗结核药物;②纯蛋白衍生物(PPD)皮肤试验阳性,胸片正常与未使用过抗痨药物
The rapid diagnosis of tuberculosis is still mainly rely on optical microscopy sputum acid-fast bacilli, however, only 1/2 ~ 3/4 cases of sputum smear positive. Evidence of immune activation from peripheral blood cells suggests that M. tuberculosis usually enters the bloodstream of its sensitizing effector cells. The results of polymerase chain reaction (PCR) detection of blood samples from suspected TB patients are reported. Blood collected from the target: ① sputum smear confirmed active tuberculosis in 8 cases, were not used anti-tuberculosis drugs; ② pure protein derivative (PPD) skin test was positive, normal and chest X-ray anti-tuberculosis drugs