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目的 探讨非小细胞肺癌患者外周血淋巴细胞中CD 44表达与其临床病理特征的关系。方法 应用流式细胞术对5 0例肺癌患者外周血淋巴细胞中CD 44表达进行荧光免疫检测 ,通过自身对照分析治疗前后的变化 ,并与正常对照组 (n =2 0 )及肺部良性病变组 (n =2 5 )进行对比研究。结果 CD44在 3组中均有不同程度的表达。 5 0例肺癌患者外周血淋巴细胞中CD 44表达明显高于正常对照组及良性病变组 (P <0 .0 1)。良性病变组和正常对照组CD 44的表达无显著性差异 (P >0 .0 5 )。手术前外周血淋巴细胞中CD 44表达显著高于手术后 (P <0 .0 1) ;肺癌伴淋巴结转移者CD 44表达高于不伴淋巴结转移者 (P <0 .0 1) ;Ⅲ、Ⅳ期和Ⅰ～Ⅱ期之间CD44的表达有非常显著性差异 (P <0 .0 1) ;肺癌组织学分级与CD 44表达有明显相关性 (P <0 .0 5或P <0 .0 1) ;肺癌患者外周血中CD 44的表达与组织学类型无相关性。结论 应用流式细胞仪检测CD 44的表达水平可作为判断肺癌负荷状况、转移及预后的指标
Objective To investigate the relationship between the expression of CD44 in peripheral blood lymphocytes and the clinicopathological features in patients with non-small cell lung cancer. Methods Flow cytometry was used to detect the expression of CD44 in peripheral blood lymphocytes of 50 patients with lung cancer. Fluorescence immunoassay was used to analyze the changes of CD44 before and after treatment, and compared with the normal control group (n = 20) and benign lung diseases Group (n = 25) for comparative study. Results CD44 in all three groups had different degrees of expression. The expression of CD44 in peripheral blood lymphocytes in 50 lung cancer patients was significantly higher than that in normal controls and benign lesions (P <0.01). There was no significant difference in the expression of CD44 between the benign and normal controls (P> 0.05). The expression of CD44 in peripheral blood lymphocytes before operation was significantly higher than that after operation (P <0.01). The expression of CD44 in patients with lung cancer with lymph node metastasis was higher than that without lymph node metastasis (P <0.01) There was a significant difference in the expression of CD44 between stage Ⅳ and stage Ⅰ ~ Ⅱ (P <0.01). There was a significant correlation between the histological grade and the expression of CD44 (P <0.05 or P <0.05). 0 1). The expression of CD44 in peripheral blood of patients with lung cancer had no correlation with histological type. Conclusion The detection of CD44 expression by flow cytometry can be used as an indicator of lung cancer load status, metastasis and prognosis