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目的 研究慢性乙型肝炎患者外周血树突状细胞 (DC)的免疫功能。方法 从慢性乙型肝炎患者外周血中分离单个核细胞 ,用无血清培养法分离培养DC ,用流式细胞仪检测DC表型 ,用液闪计数仪观察DC对T细胞的增殖作用 ,用ELISA法检测混合淋巴细胞反应 (MLR)中细胞因子的分泌水平。结果 患者组DC的CD86的表达率为 (70 .2± 5 .2 ) % ,明显低于正常人组 (95 .3±3.8) % ,P <0 .0 1;其诱导T细胞增殖能力每分钟液闪计数cpm为 10 0 0 0± 2 0 0 0 ,明显低于正常人组(cpm为 30 0 0 0± 30 0 0 ) ,P <0 .0 1;患者MLR中IL 12为 (12 0 .0± 19.7) pg/ml,γ 干扰素为 (799.0± 16 1.3) pg/ml,明显低于正常人组的 (2 80 .0± 41.1) pg/ml和 (335 9.0± 6 35 .4) pg/ml,P <0 .0 1。 结论 慢性乙型肝炎患者外周血DC免疫功能低下 ,并与DC表面CD86的表达率下降及DC分泌IL 12减少密切相关
Objective To study the immune function of peripheral blood dendritic cells (DCs) in patients with chronic hepatitis B Methods Mononuclear cells were isolated from the peripheral blood of patients with chronic hepatitis B. The DCs were isolated and cultured by serum-free culture. The DC phenotypes were detected by flow cytometry. The proliferation of T cells was observed by liquid scintillation counter. Method to detect cytokine secretion in mixed lymphocyte reaction (MLR). Results The expression of CD86 in DC was (70.2 ± 5.2)%, which was significantly lower than that in normal controls (95.3 ± 3.8%), P <0.01. Minute scintillation count cpm was 10 0 0 ± 20 0 0, significantly lower than the normal group (cpm 30 0 0 0 ± 30 0 0, P <0 01; 0. ± 19.7) pg / ml and interferon - γ (799.0 ± 16 1.3) pg / ml, which was significantly lower than that of the normal control group (280 ± 41.1) pg / ml and (335 9.0 ± 6 35), respectively. 4) pg / ml, P <0.01. Conclusion The immune function of peripheral blood in patients with chronic hepatitis B is low, and it is closely related to the decrease of the expression of CD86 and the decrease of IL 12 secretion by DC