对于在生命初期参与免疫反应的免疫信号目前尚未被完全阐明。关于新生儿细胞的特点,人们对有关调节炎症基因和细胞因子产生的转录因子核因子kappa B (NF-κB)的活性目前仍知之甚少。这项研究的目的是明确人脐血单核细胞(CBMC)的NF-κB活化的特点。笔者分析了在早期免疫系统中NF-κB活性与淋巴细胞增生的潜在联系以及对细胞因子分泌的影响。为了确定遗传对新生儿免疫的影响,笔者评价了母亲的过敏反应对NF-κB调节及细胞因子分泌的影响。来自于健康新生儿的CBMC被提纯并用有丝分裂原(n=28)激活, 用电泳方法测定核提取物,用ELISA方法测定细胞因子分泌,用FISH分析排除相关的缘于母亲对CBMC的污染。所有样本均显示了确定的淋巴细胞增生反应,并且经过有丝分裂原刺激后NF-κB活性均有升高和降低。在未受刺激的CBMC中,NF-κB活性增加与TNF The immune signals that are involved in the immune response in the early stages of life have not yet been fully elucidated. With regard to the characteristics of neonatal cells, little is known about the activity of the transcription factor nuclear factor kappa B (NF-κB) that regulates inflammatory genes and cytokines. The purpose of this study was to characterize the activation of NF-κB in human cord blood mononuclear cells (CBMCs). The authors analyzed the potential association of NF-κB activity with lymphoproliferation and the effects on cytokine secretion in the early immune system. In order to determine the genetic effects on neonatal immunity, the authors evaluated the effects of maternal allergic reactions on NF-κB regulation and cytokine secretion. CBMCs from healthy newborns were purified and activated with mitogen (n = 28), nuclear extracts were determined electrophoretically, cytokine secretion was measured by ELISA, and related parental contamination of CBMCs was excluded by FISH analysis. All samples showed definite lymphocytic proliferative responses, and both increased and decreased NF-κB activity after mitogen stimulation. In unstimulated CBMC, NF-κB activity increases with TNF