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目的:观察胎肾细胞悬液对去卵巢大鼠骨组织形态计量学参数的影响,探讨胎肾对大鼠去卵巢所致骨质疏松症的作用。方法:48只SD雌性大鼠,随机分为5组,即假手术组、模型组、雌激素组、胎肾活细胞组和胎肾死细胞组。假手术组仅手术切除腹腔少量脂肪,其余各组行去卵巢手术造模。术后12周,假手术组及模型组使用生理盐水干预,雌激素组使用尼尔雌醇混悬液干预,胎肾活细胞组使用胎肾活细胞悬液干预,胎肾死细胞组使用胎肾死细胞悬液干预。各组连续用药干预4周后,取血清行血清雌激素水平检测,取股骨远端1/3做骨病理切片,行骨组织形态计量学观测。结果:与假手术组比较,模型组雌激素水平显著降低,差异有统计学意义(P<0.01)。骨小梁排列稀疏、无序,骨小梁间隙显著增大,差异有统计学意义(P<0.01)。骨小梁密度、骨小梁厚度、骨小梁面积百分比均显著降低,差异有统计学意义(P<0.01)。与模型组比较,雌激素组、胎肾细胞各组的雌激素水平均显著升高,差异有统计学意义(P<0.01)。骨小梁排列较紧密、排列较整齐、交织成网,骨小梁间隙显著缩小,差异有统计学意义(P<0.01)。骨小梁密度、骨小梁厚度、骨小梁面积百分比均显著增加,差异有统计学意义(P<0.01)。与雌激素组比较,胎肾细胞各组雌激素水平差异无统计学意义(P>0.05),但胎肾死细胞组骨小梁间隙显著缩小且骨小梁密度显著增加,差异有统计学意义(P<0.01)。与胎肾活细胞组比较,胎肾死细胞组骨小梁间隙显著缩小、骨小梁密度显著增加,差异有统计学意义(P<0.05)。结论:胎肾细胞各组对去卵巢大鼠骨量的增加、骨小梁结构及其连接性的改善、血清雌二醇水平的提高有明显作用,说明胎肾有治疗大鼠去卵巢所致骨质疏松症的作用。
OBJECTIVE: To observe the effect of fetal renal cell suspension on bone histomorphometry in ovariectomized rats and to explore the effect of fetal kidney on osteoporosis induced by ovariectomy in rats. Methods: Forty-eight SD female rats were randomly divided into 5 groups: sham operation group, model group, estrogen group, fetal kidney live cell group and fetal kidney dead cell group. Only a small amount of fat was removed from the peritoneal cavity in the sham operation group, while the other groups were ovariectomized. At 12 weeks after operation, sham-operation group and model group were treated with saline, estrogen group with nylestriol suspension intervention, fetal kidney live cell group with fetal kidney live cell suspension intervention, fetal kidney dead cell group with fetal Dead kidney cell suspension intervention. Serum levels of serum estrogen were measured after 4 weeks of continuous medication intervention in each group. One-third of the distal femur was taken for pathological sectioning and bone histomorphometry was performed. Results: Compared with the sham operation group, the estrogen level in the model group decreased significantly (P <0.01). Sparse trabecular arrangement, disorder, trabecular bone gap was significantly increased, the difference was statistically significant (P <0.01). Trabecular density, trabecular thickness, trabecular area percentage were significantly decreased, the difference was statistically significant (P <0.01). Compared with the model group, estrogen group and fetal kidney cell group estrogen levels were significantly increased, the difference was statistically significant (P <0.01). Trabecular arrangement closer, arranged neatly, intertwined into mesh, trabecular clearance was significantly reduced, the difference was statistically significant (P <0.01). Trabecular density, trabecular thickness, trabecular area percentage were significantly increased, the difference was statistically significant (P <0.01). Compared with the estrogen group, there was no significant difference in the levels of estrogen between the fetal kidney cell groups (P> 0.05), but the trabecular bone gap was significantly reduced and the trabecular bone density was significantly increased in the fetal kidney dead cells group, the difference was statistically significant (P <0.01). Compared with the fetal kidney live cell group, the trabecular bone gap was significantly reduced in the fetal kidney dead cell group, and the trabecular bone density was significantly increased (P <0.05). Conclusion: The fetal kidney cells in each group increased the amount of bone in ovariectomized rats, trabecular structure and connectivity improved, serum estradiol levels have a significant effect, indicating that the treatment of fetal kidney ovariectomy The role of osteoporosis.