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目的 研究p53、p2 1ras、nm2 3 H1基因在子宫内膜癌中的表达与临床病理学关系。方法 应用免疫组织化学方法 (SABC法 )研究 18例正常增生期子宫内膜、12例子宫内膜非典型性增生过长、78例子宫内膜癌的p53、p2 1ras、nm2 3 H1基因的表达情况。结果 12例增生期子宫内膜中p53和p2 1ras基因表达阴性 ,3例子宫内膜非典型性增生过长中p53阳性 ;p53、p2 1ras、nm2 3基因在子宫内膜癌中表达率分别为 57 69% ,55 12 % ,71 79% ;p53的表达在不同的临床分期 ,病理学分级 ,有无淋巴结转移和是否绝经之间差异有显著性意义 (P <0 0 1) ;p2 1ras基因表达与病理学分级及有无淋巴结转移有关 (P <0 0 5) ,但与临床分期无关 ;nm2 3 H1的表达与病理学分级 ,临床分期 ,有无淋巴结转移有关。结论 p53、p2 1ras、nm2 3基因在子宫内膜癌中均高表达 ,p53的表达和肿瘤发生 ,发展有关 ,p2 1ras基因表达与肿瘤的进展有关 ,nm2 3的表达与淋巴结转移呈负相关。
Objective To investigate the relationship between the expression of p53, p21ras, nm23H and endometrial carcinoma in clinicopathology. Methods Immunohistochemistry (SABC method) was used to study the expression of p53, p2 1 ras and nm2 3 H1 genes in 18 cases of normal proliferative endometrium, 12 cases of endometrial atypical hyperplasia and 78 cases of endometrial carcinoma Happening. Results The expression of p53 and p21ras genes was negative in proliferative endometrium and p53 was positive in 3 cases of endometrial atypical hyperplasia. The expression rates of p53, p21ras and nm23 in endometrial carcinoma were 57 69%, 55 12%, 71 79%. There was a significant difference in the expression of p53 between different clinical stage, pathological grade, lymph node metastasis and menopause (P <0.01) The expression was related to pathological grade and lymph node metastasis (P <0 05), but not to clinical stage. The expression of nm23 H1 was related to pathological grade, clinical stage and lymph node metastasis. Conclusions The expressions of p53, p2 1 ras and nm23 genes are all highly expressed in endometrial carcinoma. The expression of p53 is related to the development of tumor. The expression of p2 1 ras gene is related to tumor progression. The expression of nm23 protein is negatively correlated with lymph node metastasis.