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目的 验证肺癌中端粒酶活性的存在 ,探寻支气管镜活检标本的端粒酶活性测定在肺癌诊断中的价值。方法 以端粒重复扩增技术 (TRAP)分析手术标本及支气管镜活检标本的细胞提取液中端粒酶的活性。TRAP分析的结果与病理检查结果进行比较。结果 10例肺癌手术标本中均检测到端粒酶活性。 10例手术获得的正常肺组织中没有检测到端粒酶活性。 11例肺癌支气管镜活检标本中有 8例检测到端粒酶活性。 3例肺良性病变支气管镜活检标本中有 1例检测到端粒酶活性。 12例支气管镜活检获得的正常支气管粘膜标本中有 3例检测到端粒酶活性。结论 肺癌组织中有可检测到的端粒酶活性。检测支气管镜活检标本中端粒酶的活性可能有助于肺癌的诊断。
Objective To verify the existence of telomerase activity in lung cancer and to explore the value of telomerase activity in bronchoscopic biopsy in the diagnosis of lung cancer. Methods The telomerase activity in the cell extracts of surgical specimens and bronchoscopic biopsies was analyzed by telomeric repeat amplification (TRAP). The results of the TRAP analysis were compared with the pathological findings. Results The telomerase activity was detected in 10 cases of lung cancer. No telomerase activity was detected in 10 of the normal surgically-obtained lung tissues. Telomerase activity was detected in 8 of 11 lung cancer bronchoscopic biopsies. Telomerase activity was detected in 1 of 3 lung biopsy specimens. Telomerase activity was detected in 3 of the 12 cases of normal bronchial mucosa obtained from bronchoscopic biopsy. Conclusion There is detectable telomerase activity in lung cancer. Detection of telomerase activity in bronchoscopic biopsies may be helpful in the diagnosis of lung cancer.