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采用反相离子对高效液相色谱法，以洛美沙星作内标，测定人血浆、胆汁、尿中左氧氟沙星的含量。以甲醇沉淀血浆中的蛋白质，上清液６０℃氮气吹干，流动相溶解进样；胆汁和尿分别用流动相和水稀释后进样。经μ－ＢｏｎｄａｐａｋＣ１８柱分离，流动相为０．０２ｍｏｌ／Ｌ磷酸盐缓冲液－甲醇－０．５ｍｏｌ／Ｌ四丁基溴化铵（体积比为７０２５５，ｐＨ２．５６），流速１．０ｍｌ／ｍｉｎ，检测波长２９４ｎｍ。血浆、胆汁和尿中药物最低检测浓度分别为０．０５５、０．１３７５和０．０５５μｇ／ｍｌ；平均回收率分别为９８．５１％、９６．９４％、１０３．４９％；日内相对标准差为０．５２％～８．４１％，日间相对标准差为０．７９％～９．６９％。用本法对１１例临床病例进行了药物浓度测定。
Using reversed-phase ion-pair high performance liquid chromatography with lomefloxacin as internal standard, the contents of levofloxacin in human plasma, bile and urine were determined. The protein in plasma was precipitated with methanol. The supernatant was dried under nitrogen at 60 ° C and the mobile phase was dissolved into the sample. The bile and urine samples were diluted with mobile phase and water, respectively. The mobile phase was 0.02mol / L phosphate buffer-methanol-0.5mol / L tetrabutylammonium bromide (volume ratio 70255, pH 2.56) and the flow rate was 1.0ml / min , Detection wavelength 294nm. The lowest detectable concentrations of drugs in plasma, bile and urine were 0.055, 0.1375 and 0.055 μg / ml, respectively. The average recoveries were 98.51%, 96.94% and 103.49% respectively. The relative standard deviations 0.52% ~ 8.41%, daytime relative standard deviation was 0.79% ~ 9.69%. Using this method on 11 cases of clinical drug concentration determination.