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目的研究黄芪注射液拮抗氯化镉诱导的大鼠睾丸和附睾毒性作用及其机制。方法将50只SpragueDawley雄性大鼠随机分成5组:低浓度黄芪组(A1)、高浓度黄芪组(A2)、环磷酰胺组(CP)、氯化镉组(Cd)和对照组(C)。预先连续7d分别给A1组和A2组大鼠腹腔注射黄芪注射液5g·kg-1·d-1和10g·kg-1·d-1,CP组和Cd组以等量蒸馏水腹腔注射作对照。之后连续21d给A1组、A2组和Cd组大鼠同时腹腔注射氯化镉溶液(0.2mg·kg-1·d-1)。染镉后第22天处死大鼠,观察睾丸脏器系数、精子头计数、每日精子生成量、附睾尾精子计数和畸形率以及睾丸和附睾病理学;检测血清和睾丸组织总超氧化物歧化酶(T-SOD)和丙二醛(MDA)。结果A2组睾丸脏器系数、睾丸精子头计数、每日精子生成量和附睾精子计数[(5.68±1.19)、(49±9)×106/g、(10.2±2.3)×106/g、(47.5±22.5)×106/ml]明显高于Cd组[(3.11±0.16)、(39±11)×106/g、(5.1±0.3)×106/g、(10.9±2.4)×106/ml](P<0.05或P<0.01);A2组大鼠精子畸形率[(7.04±0.12)%]明显下降,与Cd组[(17.81±1.55)%]相比,差异有非常显著性(P<0.01);A2组血清及睾丸组织MDA含量和T-SOD活力与Cd组比较差异均有非常显著性(P<0.01)。结论黄芪注射液可用于防治氯化镉的生殖毒性作用,其作用机制可能与黄芪清除氧自?
Objective To study the antagonism of Huangqi injection on the toxic effects of cadmium chloride on rat testis and epididymis and its mechanism. Methods A total of 50 Sprague Dawley male rats were randomly divided into 5 groups: low concentration Astragalus group (A1), high concentration Astragalus group (A2), cyclophosphamide group (CP), cadmium chloride group (Cd) and control group (C) . Rats in group A1 and group A2 were injected intraperitoneally with Astragalus Injection 5g·kg-1·d-1 and 10g·kg-1·d-1 respectively for 7 days in advance. The rats in CP group and Cd group were given intraperitoneal injection of distilled water as control. . After 21 days, A1 group, A2 group and Cd group rats were given intraperitoneal injection of cadmium chloride solution (0.2 mg·kg-1·d-1). Rats were sacrificed on day 22 after cadmium staining. The testicular organ coefficient, sperm head count, daily sperm production, epididymal sperm count and deformity rate, and pathology of testis and epididymis were observed; total superoxide dismutase was detected in serum and testis tissue. Enzymes (T-SOD) and malondialdehyde (MDA). Results The testicular organ coefficient, testicular sperm head count, daily sperm production and epididymal sperm count in group A2 were [(5.68±1.19), (49±9)×106/g, (10.2±2.3)×106/g, ( 47.5±22.5)×106/ml was significantly higher than Cd group[(3.11±0.16),(39±11)×106/g,(5.1±0.3)×106/g,(10.9±2.4)×106/ml ] (P<0.05 or P<0.01); Sperm abnormality rate in the A2 group [(7.04±0.12)%] decreased significantly, compared with the Cd group [(17.81±1.55)%], the difference was very significant (P) <0.01); A2 group serum and testicular tissue MDA content and T-SOD activity compared with Cd group were significantly different (P <0.01). Conclusion Astragalus injection can be used to prevent and treat the reproductive toxicity of cadmium chloride. Its mechanism may be related to the scavenging of oxygen by Astragalus mongholicus.