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为探明仓储自然醇化片烟真菌的群落多样性,采用MiSeq高通量测序技术对云南沾益、贵州长顺、福建尤溪、河南确山的上、中、下3个部位共12份自然醇化片烟样品中真菌ITS进行分析.结果表明:①12份样品共获得1155个操作分类单元(Operational taxonomic unit,OTU),真菌群落丰富度和多样性最高的样品分别是福建尤溪B2F和C3F自然醇化片烟.②12份样品的优势菌门均为子囊菌门(Ascomycota).云南沾益C3F、X2F自然醇化片烟优势菌属为茎点霉属(Phoma);河南确山B2F、C23F、X2F自然醇化片烟优势菌属为链格孢属(Alternaria);贵州长顺X23F自然醇化片烟的优势菌属为Monographella;其余6份样品的优势菌属均为曲霉属(Aspergillus).③上、中、下部叶4个产地共有的OTU个数分别为121、79、168个,共有OTU的reads数占各样品OTU总reads数的70%以上(除福建尤溪C3F外),同一部位不同产地自然醇化片烟真菌群落结构差异主要体现在优势菌属的占比方面.“,”To investigate the diversity of fungal community in tobacco strips during aging, the fungal ITS DNA in 12 samples of upper, middle and lower stalk leaves from four growing areas (Zhanyi in Yunnan, Changshun in Guizhou, Youxi in Fujian and Queshan in Henan) were sequenced by MiSeq. The results showed that: 1) A total of 1 155 operational taxonomic units (OTUs) were obtained from the 12 samples. Samples B2F and C3F from Youxi had the highest fungal community richness and the most abundant biodiversity, respectively. 2) Ascomycota was the dominant phyla in the 12 samples. Phoma, Alternaria and Monographella were the dominant genera in samples C3F and X2F from Zhanyi, samples B2F, C23F and X2F from Queshan and sample X23F from Changshun. Aspergillus was the dominant genera in the other 6 samples. 3) 121, 79 and 168 of OTUs were common to the samples of upper, middle and lower stalk leaves from the 4 areas. The number of reads from the common OTUs accounted for more than 70% of the total number of reads in each sample, except for the sample C3F from Youxi. The differences in fungal community structure from the same stalk position of different areas mainly reflected in the proportion of dominant genera.