论文部分内容阅读
目的探索黄腐酚对人非小细胞肺癌细胞株A549与H1650凋亡的影响。方法采用CCK-8法检测黄腐酚对A549与H1650细胞增殖的影响;流式细胞术分析黄腐酚对两种细胞凋亡的影响;Western Blot法检测两种细胞被不同浓度黄腐酚干预后细胞中凋亡相关蛋白Bax、Bcl-xl、pro-caspase-3的表达。结果黄腐酚能有效抑制A549与H1650细胞的增殖,其抑制强度与浓度呈正相关,对H1650细胞有更好的抑制作用;两种细胞的凋亡率随黄腐酚浓度的增大而上升,5μmol·L-1诱发的凋亡率与同浓度顺铂无差异(P>0.05);两种细胞中凋亡相关蛋白的表达与黄腐酚浓度相关。结论黄腐酚可能通过调控Bcl-xl、Bax、Caspase-3及其相关基因使非小细胞肺癌细胞发生凋亡。
Objective To explore the effects of xanthohumol on the apoptosis of human non-small cell lung cancer cell lines A549 and H1650. Methods The effects of xanthohumol on the proliferation of A549 and H1650 cells were determined by CCK-8 assay. The effects of xanthohumol on the apoptosis of the two cell lines were analyzed by flow cytometry. Western Blot was used to detect the effects of xanthohumol on the proliferation of A549 and H1650 cells. The expression of Bax, Bcl-xl and pro-caspase-3 in post-cell. Results The results showed that xanthohumol could effectively inhibit the proliferation of A549 and H1650 cells. The inhibitory effect of xanthohumol on the proliferation of H1650 cells was better than that of H1650 cells. The apoptosis rate of both cells increased with the increase of xanthohumol concentration. The apoptosis rate induced by 5μmol·L-1 was not different from cisplatin at the same concentration (P> 0.05). The expression of apoptosis-related proteins in both cells correlated with the concentration of xanthohumol. Conclusion Xanthohumol may induce apoptosis of non-small cell lung cancer cells through the regulation of Bcl-xl, Bax, Caspase-3 and their related genes.