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本文报道了家蚕细胞质多角体病毒(简称CPV)颗粒经紫外光照射后,通过DEAE-Sephadex A-25 柱层析,用氯化钠溶液分步洗脱分离得到基因-酶复合物。以~3H-UTP和[~3H-甲基]-S-腺苷-L-甲硫氨酸(SAM)作为底物时,该复合物均呈现RNA多聚酶和甲基转移酶活力。与CPV的RNA基因相同,基因-酶复合物能由聚丙烯酰胺凝胶电泳分成9条片段,各个片段也均呈现RNA多聚酶和甲基转移酶的酶活力。说明CPV颗粒里的RNA多聚酶和甲基转移酶紧密结合于双链RNA基因上,在复制过程中,CPV的双链RNA基因的每一片段各自独立地进行转录。
This paper reports the silkworm cytoplasmic polyhedrosis virus (CPV) particles after UV irradiation, by DEAE-Sephadex A-25 column chromatography, sodium chloride solution step by step to elute gene-enzyme complex. With ~ 3H-UTP and [~ 3H-methyl] -S-adenosyl-L-methionine (SAM) as substrates, the complex exhibits both RNA polymerase and methyltransferase activities. In the same way as the CPV RNA genes, the gene-enzyme complex can be separated into 9 fragments by polyacrylamide gel electrophoresis and each fragment also exhibits the activity of RNA polymerase and methyltransferase. Indicating that RNA polymerase and methyltransferase in CPV particles are tightly bound to the double-stranded RNA gene. Each fragment of the double stranded RNA gene of CPV is independently transcribed during replication.