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为比较室内药效的不同测定方法,在实验室内活体和离体条件下,采用菌片接种法测定不同化学药剂(500 g/L异菌脲悬浮剂、50%福美双可湿性粉剂、50%啶酰菌胺水分散粒剂、70%甲基硫菌灵可湿性粉剂)对黄瓜灰霉病的保护作用和治疗作用,在含药培养基上,采用菌丝生长速率法测定不同化学药剂对黄瓜灰霉病菌菌丝生长的抑制作用。结果表明,化学药剂的活体效果优于离体效果,但离体条件下的药效趋势与活体条件下的药效趋势一致,说明可用离体试验代替活体试验;化学药剂在培养基上的效果优于在寄主组织上的药效,但利用寄主组织进行药效试验与田间药效试验更近了一步,准确度更高,说服力更强。
In order to compare different determination methods of indoor efficacy, different chemical agents (500 g / L ivermectin suspension, 50% thiram, 50 % Boscalid water dispersible granules, 70% thiophanate-methyl wettable powder) on the cucumber gray mold disease and the protective effect on the drug-containing medium, mycelial growth rate method for the determination of different chemical agents Inhibitory effect on the mycelial growth of Botrytis cinerea cucumber. The results showed that the in vivo effect of chemical agents was better than the in vitro effect, but the drug efficacy trend under in vitro conditions was consistent with the drug efficacy trend under in vivo conditions, indicating that the in vitro test can replace the in vivo test; the effect of chemical agents on the medium Which is superior to that of the host tissue. However, using the host tissue for the efficacy test is one step closer to the field efficacy test, with higher accuracy and convincing power.