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以9个藜麦(Chenopodium quinoa Willd.)品种为材料,对藜麦茎段、子叶不同外植体的愈伤组织诱导效果进行比较,同时对茎段的愈伤组织诱导以及愈伤组织增殖体系进行优化试验。结果表明:诱导愈伤组织最佳外植体为茎段,9个品种在培养基MS+0.5 mg/L2,4-D中用茎段诱导愈伤组织的平均诱导率达90%;在愈伤组织诱导优化试验中,处理Ⅵ(MS+0.5 mg/L 2,4-D+0.5 mg/L KT+0.5 mg/L NAA)和处理Ⅱ(MS+0.5 mg/L 2,4-D)对藜麦愈伤组织的诱导率相近,但是愈伤形态差别较大,后者诱导形成疏松、有光泽、黄白色愈伤组织,因此MS+0.5 mg/L 2,4-D培养基为最佳愈伤组织诱导培养基;2,4-D与KT、NAA搭配使用时,愈伤组织增殖速率明显上升。
Nine varieties of Chenopodium quinoa Willd. Were used as materials to compare the induction effects of different explants on the stem segments and cotyledons of the quinoa. Meanwhile, the callus induction of the stem segments and callus proliferation system Optimize the test. The results showed that the best explants of induced callus were the stem segments. The average induction rate of callus induced by stem segments in 9 cultivars was 90% in medium MS + 0.5 mg / L 2,4-D. In the tissue culture optimization experiment, the effects of Ⅵ (MS + 0.5 mg / L 2,4-D + 0.5 mg / L KT + 0.5 mg / L NAA) and treatment Ⅱ (MS + 0.5 mg / L 2,4-D) The callus induction rate of quinoa was similar to that of callus, but the callus morphology varied greatly. The latter induced the formation of loose, glossy, yellow-white callus. Therefore, MS + 0.5 mg / L 2,4-D medium was the most Good callus induction medium; 2,4-D with KT, NAA with the use of the callus proliferation rate increased significantly.