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目的探讨室内外空气污染对学龄儿童外周血淋巴细胞凋亡早期的影响。方法于2008年4—5月运用现场采样结合实验室检测调查太原市两路口空气污染状况,选取其中重交通污染区小学生94名和轻交通污染区45名作为研究对象,采用调查表调查个人一般情况、与室内污染相关的因素以及家居环境状况。采用Annexin-V-FITC/PI联合探针染色,流式细胞方法检测学龄儿童淋巴细胞处于凋亡早期的百分率(经对数转换接近正态分布)。结果单因素分析发现,重交通污染区学龄儿童淋巴细胞凋亡早期水平为(4.397±5.514)%,轻交通污染区学龄儿童淋巴细胞凋亡早期水平为(2.099±1.137)%,两者差异有统计学意义(P<0.05)。重污染区被动吸烟组凋亡早期水平为(2.802±1.421)%,高于轻污染区被动吸烟组[(1.871±0.403)%],且高于重污染区无被动吸烟组[(2.499±1.148)%],差异有统计学意义(P<0.05)。轻污染区一年内无装修组凋亡早期水平为[(1.979±0.422)%],低于重污染区无装修组[(2.648±1.223)%],且低于重污染区装修组[(2.954±1.858)%],重污染区装修组凋亡早期水平高于重污染区无装修组,差异有统计学意义(P<0.05)。结论交通污染、被动吸烟、室内装修均可诱导学龄儿童淋巴细胞凋亡早期水平增加。
Objective To investigate the effects of indoor and outdoor air pollution on the early apoptosis of peripheral blood lymphocytes in school-age children. Methods From April to May 2008, the spot sampling and laboratory tests were used to investigate the air pollution status of the two junctions in Taiyuan City. 94 primary school students and 45 light pollution areas were selected as the research objects. The questionnaire was used to investigate the general situation of individuals , Factors related to indoor pollution and the status of home environment. Annexin-V-FITC / PI combined probe staining, flow cytometry method was used to detect the percentage of lymphocytes in school-age children in the early stage of apoptosis (logarithmic transformation close to normal distribution). Results Univariate analysis showed that the incidence of lymphocyte apoptosis in school-age children in heavy traffic pollution was (4.397 ± 5.514)% at early stage, and (2.099 ± 1.137)% at school-age children in light traffic pollution. There was a significant difference between the two groups Statistical significance (P <0.05). The level of apoptosis in passive smoking group was (2.802 ± 1.421)% higher than that in passive smoking group [(1.871 ± 0.403)%] in heavy pollution area and higher than that in passive smoking group [(2.499 ± 1.148 )%], The difference was statistically significant (P <0.05). In the lightly contaminated area, the level of early apoptosis in the no-decoration group was [(1.979 ± 0.422)%] in one year, lower than that in the heavily-contaminated area [(2.648 ± 1.223)%] ± 1.858)%]. The levels of apoptosis in the heavily contaminated area renovation group were higher than those in the heavily contaminated area, the difference was statistically significant (P <0.05). Conclusions Traffic pollution, passive smoking, and interior decoration can all induce the early stage of lymphocyte apoptosis in school-age children to increase.