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目的： 建立超临界流体萃取（ＳＦＥ） 技术和毛细管气相色谱（ＣＧＣ） 非在线联用技术测定中成药白蚀丸和白癜风胶囊中的补骨脂素和异补骨脂素的含量。方法： 采用均匀设计法和多元回归分析确定影响超临界流体萃取效率的主效应及适宜的操作条件并以ＣＧＣ 法进行定量。结果： 白蚀丸和白癜风胶囊分别含补骨脂素０－１２１ ％ 及０－０５５ ％ ， 异补骨脂素０－１２５ ％ 及０－０３４ ％ ， 结论： ＳＦＥ－ＣＧＣ 法测定白蚀丸和白癜风胶囊中有效成分快速可靠、准确灵敏。
OBJECTIVE: To establish a method for the determination of psoralen and isopsoralen in proprietary Chinese medicine Baixue Pills and Vitiligo Capsules by using supercritical fluid extraction (SFE) and capillary gas chromatography (CGC) off-line combination technology. Methods: The main effects and the suitable operating conditions affecting the extraction efficiency of supercritical fluid were determined by uniform design and multiple regression analysis and quantified by CGC method. Results: The white eclipse pills and vitiligo capsules contained 0-121% and 0-055% of psoralen, 0-125% and 0-034% of isopsoralen respectively. Conclusion: The results of SFE-CGC method for determination of white eclipse pills and vitiligo capsules Active ingredients in the fast and reliable, accurate and sensitive.