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一种新的冠状病毒SARS-CoV是引起严重急性呼吸综合征(severe acute respiratory syndrome,SARS)的病原体,对由SARS病毒全基因组序列推导出的所有蛋白质逐一进行分子量、等电点、分子消光系数等物理化学性质计算,以及跨膜区和亚细胞定位预测,辅以保守序列家族数据库搜索,预测SARS-CoV功能未知蛋白质的功能,同时,通过SARS-CoV与其他冠状病毒蛋白质同源序列比较和进化距离计算,分析SARS病毒的分类地位以及与其他冠状病毒的进化关系。结果表明,尽管SARS病毒是不同于其他3组冠状病毒的一种全新冠状病毒,但在进化关系上更靠近牛冠状病毒BoCoV和鼠肝炎病毒MHV。为实验测定SARS病毒蛋白质组以及抗SARS疫苗研制提供了参考和帮助。
A new coronavirus, SARS-CoV, is the causative agent of severe acute respiratory syndrome (SARS). The molecular weight, isoelectric point and molecular extinction coefficient of all the proteins deduced from the whole genome sequence of SARS virus CoV and other coronavirus protein homologous sequences were compared by SARS-CoV and other coronavirus protein sequences, and the results of physical and chemical properties calculations, transmembrane region and subcellular localization prediction, combined with the conserved sequence family database search, prediction of SARS-CoV function of unknown proteins, Evolutionary distance calculations to analyze the taxonomic status of the SARS virus and the evolutionary relationships with other coronaviruses. The results showed that, although the SARS virus is a new coronavirus different from the other three groups of coronaviruses, the evolutionary relationship is closer to the bovine coronavirus BoCoV and the murine hepatitis virus MHV. It provides reference and help for the experimental determination of SARS virus proteome and the development of anti-SARS vaccine.