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目的 建立ｂＦＧＦ的检测方法并检测直肠癌患者癌组织及癌旁正常组织中的含量。方法 用纯化的ｂＦＧＦ免疫白兔制得抗血清，再用Ｗｅｓｔｅrｎ ｂｌｏｔ方法检测组织中的ｂＦＧＦ含量。结果 ①癌组织中ｂＦＧＦ的含量显著高于正常组织；②无淋巴转移时癌组织中ｂＦＧＦ含量与癌旁组织有显著差异，而有淋巴转移时癌组织中ｂＦＧＦ含量与癌旁组织有差异。结论 直肠癌组织中ｂＦＧＦ含量明显升高且癌旁组织bFGF升高与肿瘤是否有淋巴转移有关。
Objective To establish the detection method of bFGF and detect the content of cancer tissue and adjacent normal tissues of rectal cancer patients. Methods Anti-serum was prepared by immunizing rabbits with purified bFGF, and the content of bFGF in tissues was detected by Western blot. Results 1 The content of bFGF in cancer tissues was significantly higher than that in normal tissues. 2 The content of bFGF in cancer tissues was significantly different from that in adjacent tissues in the absence of lymphatic metastasis, while the content of bFGF in tissues with lymphatic metastases was different from that in adjacent tissues. Conclusion The bFGF content in rectal cancer tissue is significantly elevated and the increase of bFGF in the adjacent tissue is related to lymph node metastasis.