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目的探讨ＲＮＡＤＮＡ含量及细胞增殖指数（ＰＩ）在原发性肝细胞癌（ＰＨＣ）中的应用价值．方法应用流式细胞分析技术，对６４例（男４６例，女１８例，年龄２４岁～７０岁，平均５１５岁）ＰＨＣ细胞ＲＮＡ，ＤＮＡ含量及ＰＩ进行了定量分析，并对患者进行了１年～５５年的随访．结果ＲＮＡ和ＤＮＡ指标对ＰＨＣ的诊断符合率分别为９３７％和７５０％，双指标联合应用诊断符合率为９６８％，ＲＮＡ指标更敏感．ＲＮＡ和ＤＮＡ含量与预后关系密切．ＤＮＡ含量随组织学分级的增高而增加（Ⅰ级为１２５，Ⅱ级为１４０，Ⅲ级为１５１，Ⅳ级为１６８），组织学各级之间ＤＮＡ含量均有显著性差异（Ｐ＜００５），提示ＤＮＡ含量可作为组织学分级的定量指标．ＤＮＡ倍体与年龄（＜５０岁，８２９％ｖｓ≥５０岁，６０９％；Ｐ＜００５）、ＨＣＶ感染（８１２％ｖｓ５６３％，Ｐ＜００５）密切相关，与性别、ＡＦＰ水平、ＨＢｓＡｇ及瘤体大小无关．ＰＩ值显著高于正常对照组（０３８±０１２ｖｓ０１０±００４，Ｐ＜００１），ＰＩ＞０４０者预后差．结论ＲＮＡ，ＤＮＡ含量及ＰＩ值可作为ＰＨＣ的诊断及判断预后的指标，ＲＮＡ及ＤＮＡ双指标联合应用价值更?
5. Objective To explore the value of RNA DNA content and cell proliferation index (PI) in primary hepatocellular carcinoma (PHC). Methods Flow cytometric analysis was used to quantitatively analyze RNA and DNA levels and PI in 64 patients (46 males and 18 females, aged 24 to 70 years, mean 51 to 5 years of age) in PHC cells. 1 to 5 to 5 years of follow-up. Results The diagnostic accuracy rates of RNA and DNA markers for PHC were 93.7% and 75.0%, respectively. The coincidence rate of dual-indicators combined diagnosis was 96.8%. RNA markers were more sensitive. RNA and DNA content are closely related to prognosis. DNA content increased with increasing histological grade (level I was 125, grade II was 140, grade III was 151, grade IV was 168), and histological grades had DNA content. Significant difference (P<005), suggesting that DNA content can be used as a quantitative indicator of histological grade. DNA ploidy was closely related to age (<50 years, 829% vs ≥50 years, 609%; P<005), HCV infection (812% vs563%, P<005) Related, not related to gender, AFP level, HBsAg and tumor size. The PI value was significantly higher than the normal control group (038±012vs010±004, P<001). PI>040 had poor prognosis. [Conclusion] RNA, DNA content and PI value can be used as the diagnosis of PHC and the prognosis index.