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目的:建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定益气软皮丸中黄芪甲苷含量的方法。方法:采用Agilent TC-C18色谱柱(250mm×4.6mm,5μm),以乙腈-水(32∶68)为流动相,流速为1.0mL·min-1,柱温为30℃,漂移管温度为100℃,载气流速为2.5mL·min-1,进样量为20μL。结果:黄芪甲苷在1.02~10.20μg范围内线性关系良好,线性方程为Y=1.7281 X+1.9515(r=0.9998),平均回收率为97.59%,RSD为0.95%(n=6)。结论:该方法准确、简便、重复性好,可用于该制剂中黄芪甲苷含量的测定。
OBJECTIVE: To establish a HPLC-ELSD method for the determination of Astragaloside IV in Yiqitapi pills. METHODS: The mobile phase consisted of acetonitrile-water (32:68) with a mobile phase of Agilent TC-C18 (250 mm × 4.6 mm, 5 μm) at a flow rate of 1.0 mL · min- 100 ℃, carrier gas flow rate of 2.5mL · min-1, the injection volume of 20μL. Results: Astragaloside had a good linearity in the range of 1.02-10.20μg. The linear equation was Y = 1.7281 X + 1.9515 (r = 0.9998). The average recovery was 97.59% and the RSD was 0.95% (n = 6). Conclusion: The method is accurate, simple and reproducible. It can be used for the determination of astragaloside IV in the preparation.