用光敏生物素标记利什曼原虫动基体DNA(k-DNA),以打点杂交鉴定利什曼原虫。结果表明,在前鞭毛体数为10~4～10~5时,此法能显示出利什曼原虫不同的种和株间有明显的区别,并且发现采自国内黑热病流行病学特征不同的地区的虫株间k-DNA 在同源程度上存在差异,但用传统分类法则无法加以鉴别。此法为快速、简便地鉴定利什曼原虫及诊断黑热病提供了新的途径。 Leishmania motile DNA (k-DNA) was labeled with photo-biotin, and Leishmania japonica was identified by dot blotting. The results showed that when the number of promastigotes was 10 ~ 4 ~ 10 ~ 5, this method could show a significant difference between different species and strains of Leishmania, and found that the epidemiological characteristics of Leishmania were different There are differences in the homology between k-DNA in insect strains in different regions, but they can not be identified by traditional classification methods. This method provides a new way to quickly and easily identify Leishmania and diagnose kala-azar.