A pharmacological interaction target (PIT) method for solving the difficult problem in the separation of taxol from cephalonmanine was proposed. A two-phase extraction technique was used to carry out the PIT separation process. The effects of buffer, temperature and protein on the separation were investigated. Feasible disassembly conditions were also discussed. The final purity of taxol can reach 95% or higher. A pharmacological interaction target (PIT) method for solving the difficult problem in the separation of taxol from cephalonmanine was proposed. A two-phase extraction technique was used to carry out the PIT separation process. The effects of buffer, temperature and protein on the separation were investigated. Feasible disassembly conditions were also discussed. The final purity of taxol can reach 95% or higher.