论文部分内容阅读
目的: 观察甲状腺转录因子-1 (TTF-1) 在正常成人肺泡Ⅱ型上皮细胞、人胚胎肺泡上皮细胞、肺癌以及淋巴结转移癌癌细胞胞核中的表达, 从量化角度探讨其在肺癌发生及其转移过程中的意义。方法: 石蜡包埋切片, 应用免疫组织化学SP法及LeicaQ500MC图像分析系统,对TTF- 1表达强度进行定量分析。结果: 胚胎肺泡上皮细胞胞核TTF 1阳性单位(PU) 值小于正常成人肺泡Ⅱ型上皮细胞胞核TTF- 1的PU值, 差异具有极显著性(P<0 .001); 不同类型肺癌癌细胞胞核TTF 1的PU值均小于胚胎肺泡上皮细胞和正常成人肺泡Ⅱ型上皮细胞胞核TTF -1的PU值, 且差异具有极显著性(P<0. 001); 肺腺癌和肺小细胞癌癌细胞胞核TTF 1的PU值均大于肺鳞癌和肺大细胞癌癌细胞胞核TTF 1的PU值, 且差异均具有极显著性(P<0 .001); 肺鳞癌癌细胞胞核TTF 1的PU值大于肺大细胞癌癌细胞胞核TTF- 1的PU值, 差异具有极显著性(P<0. 001)。肺腺癌、肺鳞癌和肺大细胞癌淋巴结转移灶中癌细胞胞核TTF 1的PU值均大于其癌原发灶癌细胞胞核TTF 1的PU值, 差异具有显著性(P<0 .001, P<0 .001,P<0. 05); 肺小细胞癌淋巴结转移灶癌细胞胞核与其原发灶癌细胞胞核TTF 1的PU值基本相同, 差异无显著性(P>0 .05)。有淋巴结转移的肺癌癌细胞胞核TTF 1的PU值大于无淋巴结转移
Objective: To observe the expression of thyroid transcription factor-1 (TTF-1) in the nuclei of normal adult type II alveolar epithelial cells, human embryonic alveolar epithelial cells, lung cancer and lymph node metastatic cancer cells. The significance of its transfer process. Methods: Paraffin embedded sections were used to quantitatively analyze the expression intensity of TTF-1 using immunohistochemical SP method and Leica Q500MC image analysis system. Results: The value of TTF 1 in nuclei of embryonic alveolar epithelial cells was smaller than that of TTF-1 in nuclei of type Ⅱ epithelial cells in normal alveolar epithelial cells (P <0.01). The different types of lung cancer The PU value of TTF 1 in cell nucleus was lower than that of TTF -1 in embryonic alveolar epithelial cells and normal adult type II alveolar epithelial cells, and the difference was highly significant (P <0.001). The lung adenocarcinoma and lung The PU value of TTF 1 in the small cell carcinoma cell nucleus was larger than that of the lung squamous cell carcinoma and the large cell lung cancer TTF 1, and the difference was highly significant (P <0. 001); lung squamous cell carcinoma The PU value of the nucleus of TTF 1 in cancer cells was larger than the PU value of TTF-1 in the nucleus of cancer cells. The difference was highly significant (P <0.001). The PU value of TTF 1 in lung adenocarcinoma, lung squamous cell carcinoma and lymph node metastasis of large cell lung cancer was higher than that of the TTF 1 in the nucleus of cancer cell, the difference was significant (P <0 .001, P <0.001, P <0.05). There was no significant difference in the PU value between the nuclei of lymph node metastasis of small cell lung cancer cells and the nucleus of primary tumor cells TTF 1 (P> 0 .05). The PU value of nuclear TTF 1 in lymph node metastatic lung cancer cells was larger than that in non-lymph node metastasis